Fig. 5

Different steps during meristem isolation and in vitro propagation of Fragaria chiloensis. a Meristem isolation and culture: i isolated meristem near to be cultivated, ii green meristem showing viability after culture, iii dead meristem after oxidation, iv plantlet shooting from an isolated meristem. b Morphogenic development of in vitro plantlets: i shoot induction from cultivated meristems, ii in vitro multiplication by adventitious shoot formation, iii rooting of in vitro plants. c Ex vitro adaptation of micropropagated plants: i a 35 days old plantlet ready for the ex vitro step; ii ex vitro plantlets 21 days after adaptation