Fig. 2

TUG1 knockdown reduces the apoptosis of OGD-induced hippocampal neurons. Murine hippocampal neuron cell line HT22 cells were divided into four groups: the Control group (normally cultured cells), the OGD group (cells were reoxygenated for 24 h after OGD treatment for 1 h), the OGD + LV-shRNA group (cells were transfected with LV-shRNA for 72 h before the OGD treatment), and the OGD + LV-sh-TUG1 group (cells were transfected with LV-sh-TUG1 for 72 h before the OGD treatment). a The cell viability was detected by MTT assay. b The TUG1 level was detected by qRT-PCR. c The BDNF mRNA level was detected by qRT-PCR. d, e The protein expressions of BDNF, cleaved-Caspase 3, and Bcl-2 were detected by western blotting. f, g Cell apoptosis was detected by flow cytometry. The experiments were repeated three times (n = 3). *P < 0.05 vs. the control group; **P < 0.05 vs. the OGD + LV-shRNA group