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Fig. 2 | Biological Research

Fig. 2

From: Long non‐coding RNA MALAT1 regulates cell proliferation and apoptosis via miR-135b-5p/GPNMB axis in Parkinson’s disease cell model

Fig. 2

MALAT1 knockdown promoted cell proliferation but inhibited apoptosis in MPP+-stimulated cell model of PD. a, b MPP+-stimulated SK-N-SH and SK-N-BE cells were transfected with si-NC, si-MALAT1-1, si-MALAT1-2, or si-MALAT1-3, respectively. And the expression of MALAT1 in transfected cells was assayed by qRT-PCR. (C-H) MPP+-stimulated SK-N-SH and SK-N-BE cells were transfected with si-NC or si-MALAT1-1, respectively. c, d After transfection for 0, 24, 48, or 72 h, cell viability was assessed using MTT assay. e The apoptosis rate of transfected cells was analyzed by flow cytometry assay. f, g Western blot assay was performed to determine the levels of apoptosis-related protein Bax, Cleaved-casp-3, and Bcl-2. h The protein level of PCNA was detected by western blot assay. The experiments were repeated three times (n = 3). *P < 0.05, compared to si-NC group

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