Fig. 7
From: Cisplatin-induced hydroxyl radicals mediate pro-survival autophagy in human lung cancer H460 cells
Role of hydroxyl radicals in mediating the autophagic response in human lung cancer cells evidenced with (a) up-regulation of LC3-II/LC3-I, Atg3, and Atg7 expression levels, as detected by western blot analysis, after co-culture with 100 µM hydrogen peroxide (H2O2) and 50 µM FeSO4 for 12 h, but (b) negated by pre-incubation with 1 mM deferoxamine prior to exposure to H2O2 and FeSO4. (c) Autophagosome (green fluorescence of monodansylcadaverine (MDC)) and acidic autolysosome (red fluorescence of acridine orange) formation demonstrate the autophagy stimulating effect of hydroxyl radicals (OH·) generated by co-treatment of H2O2 (100 µM) and FeSO4 (50 µM) and its inhibition by deferoxamine pretreatment. Notably, staining with acridine orange indicates green fluorescence of cytoplasm and nucleolus while bright red/orange-red fluorescence of acridine orange represents acidic compartment. Data are shown as the mean ± SD (n = 3). *p < 0.05 versus untreated control cells. #p < 0.05 versus H2O2 + FeSO4-treated cells