Fig. 4

Flow chart illustrating allocation of participants and experimental description. Beginning with 87 potential participants, 67 were excluded from the study by inclusion/exclusion criteria and the study was carried out with 20 participants. From 20, 10 were men and 10 were women. After enrollment, subjects were familiarized to the procedures and body height, mass and BP were measured before the placement of ECG leads. Then, deep breathing (DB), DB + hand grip (DB + HG) and DB + HG + circulatory occlusion (DB + HG + OC) were performed in each experimental subject