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Fig. 7 | Biological Research

Fig. 7

From: Allergens of the urushiol family promote mitochondrial dysfunction by inhibiting the electron transport at the level of cytochromes b and chemically modify cytochrome c1

Fig. 7

Litreol modifies the isoelectric point and immunoreactivity of cytochrome c1. 100 μg of protein extracted from the inner membrane from bovine heart mitochondria were separated by 2D-PAGE gels, and then protein was detected with Coomassie blue staining (left panel), radioactivity was assessed with photofluorography (middle panel), and cytochrome c1 immunoreactivity was evaluated by western blot (right panel). A magnification of the area indicated in red is shown below each panel. X, Y and Z represent the 27 kD radioactive spots with isoelectric points of 6.35, 6.19, and 6.02, respectively. 1D indicates a lane where 100 μg of protein extracts were separated only by SDS PAGE. St indicates a lane where molecular weight standards were loaded in the SDS PAGE. The gradient of pH generated (isoelectric point) is indicated

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