Table 1 Sequencing primers used in the analysis of mitochondrial genome of Alulatettix yunnanensis and Tetrix japonica
Upstream primers sequences (5′–3′) | Downstream primers sequences (5′–3′) | Anneal temperature (°C) |
|---|---|---|
190-J: AAGCTAMTGGGTTCATRCCC | 1650-F: AAYCAATTTCCGAATCCACC | 53 |
1600-J: GTTGTTGTAACAGCACATGC | 2750-F: CCTCCTATAATAGCAAATACTGCTCC | 54 |
2650-J: TTACCTGTTYTWGCWGGAGC | 3660-F: CCACAAATTTCAGAGCATTGACC | 55 |
3600-J: CAATGATACTGATCATATGAATATTC | 4900-F: ATCYCGTCATCATTGAATTAT | 53 |
4800-J: TAGTAGACTATAGTCCATGACC | 6150-F: CCATTCTTTCAGGTCGAAACTG | 55 |
5800-J: GAGCAWCTTAGGGTTATAGTT | 7600-F: TAAGWAATCGKRTWGGTGATGT | 52 |
7500-J: CAGGAGTAGGAGCAGCTATAGC | 8650-F: CTTGTAATATATCGGCTCCTCC | 56 |
8500-J: GTGTAATAAGAATAACTAATTAAGCC | 9000-F: TGTTGCAGCTTCATTACCATTATTGT | 49 |
8900-J: GGGGCCTCAACATGAGCYTT | 10600-F: TTTCATCATATTGAAATRTTTRTTGG | 51 |
10300-J: CAACAATAATGAAACAAYRAATATAG | 11600-F: AAATAYCATTCTGGTTGAATGTG | 51 |
11450-J: CCCATATATTATAGGAGAYCC | 12300-F: TATGAGTTCGGGGTACTTTACC | 53 |
12050-J: AAAAACCCCCTTCAAGCCAAAT | 13350-F: GACYGTRCAAAGGTAGCATAATC | 54 |
13150-J: TTCTCGTTAAACCTTTCATTCCAGT | 14300-F: TATTTCAGGTCAAGGTGCAGCTTAT | 54 |
14100-J: CTACTWTGTTACGACTTATCTC | 14450-F: ARACTAGGATTAGATACCCT | 51 |
14330-J: TAACATCATTCATGAAACAGGTTCCTCT | 250-F: ATTTCTAGTCCTATTCACACACCTAATC | 54 |