Fig. 2

Characteristics of CCR6 localization in normal testis. a Representative immunohistochemical images showing the expression of CCR6 in mouse and human testes (arrow). Nuclei were counterstained with hematoxylin. Enlarged images are shown in the inset; at least three independent experiments were done. Scale bar = 50 µm. b Representative immunofluorescence images showing the expression of CCR6 in mouse and human testes (arrow), respectively. Nuclei were counterstained with Hoechst 33258. Enlarged images are shown in the inset; At least three independent experiments were done. Scale bar = 50 µm. c Representative co-staining images showing the expressions of CCR6 (green) and occludin (red) in mouse testis (arrow). Nuclei were counterstained with Hoechst 33258. Enlarged images are shown in the inset; At least three independent experiments were done. Scale bar = 50 µm