Fig. 4

a Representative photographs of mice aortic arch anatomy in situ. Atherosclerotic plaque formation is notably greater in apoE^−/− mice, while no lesion was present in the age-matched WT control. Arrow heads indicates plaque deposition (4×; Bar = 2 mm). b DHE staining of representative aortic arch cross-sections. Atherosclerotic mice showed increased DHE-positive cells compared to WT (magnification 400×; Bar = 100 µm). c Quantification of ROS production. Aging increased ROS production in aortic arch cross-section and it was aggravated through hypercholesterolemia. d Representative micrographs of aortic arch stained with hematoxylin and eosin. Values are means ± SEM, **p < 0.01 vs. WT. *p < 0.05 vs. the respective young group, ^#p < 0.05 vs. age-matched WT group