Fig. 4
From: Berberine in combination with cisplatin induces necroptosis and apoptosis in ovarian cancer cells
BBR + DDP combination induced apoptosis. OVCAR3 cells (a) and POCCLs (b) were treated with BBR (100 μM) and/or DDP (5 mg/L) for 24 h. The gross changes and apoptotic morphology of OVCAR3 cells were detected by Transmission Electron Microscopy (TEM) assay under ×4200 magnification (a). The number of OVCAR3 and POCCLs undergoing apoptosis was detected by flow cytometry using Annexin V-FITC/PI double staining (b). Data were based on at least three independent experiments, and shown as mean ± SEM. *p < 0.05, **p < 0.01 compared with NC; #p < 0.05, ##p < 0.01 compared with BBR; &p < 0.05, &&p < 0.01 compared with DDP