Table 1 The details of primers sequence and the annealing temperature
Primer | Sequence (forward, reverse) | Annealing temperature |
|---|---|---|
HPRT1 | F: AATTACTTTTATGTCCCCTGTTGACTGG R: GCTATAAATTCTTTGCTGACCTGCTG | 63 |
VEGF | F: TTGCCTTGCTGCTCTACCTCCA R: GATGGCAGTAGCTGCGCTGATA | 62.5 |
HIF-1a | F: TGAGTTCGCATCTTGATAAGGC R: ACAAAACCATCCAAGGCTTTCA | 63 |
N-cadherin | F: GGACAGTTCCTGAGGGATCA R: GGATTGCCTTCCATGTCTGT | 62.5 |
Vimentin | F: GGTGGACCAGCTAACCAACGA R: TCAAGGTCAAGACGTGCCAGA | 62.5 |
β-catenin | F: AAAATGGCAGTGCGTTTAG R: TTTGAAGGCAGTCTGTCGTA | 62 |