Fig. 4
From: Palmatine ameliorates high fat diet induced impaired glucose tolerance
Effect of PAL on the MAPK signaling in palmitate (PA) treated INS-1 cells. a Cells were incubated with different doses of PAL (0 μg/ml, 10 μg/ml, 20 μg/ml, 40 μg/ml) for 1 h, 24 h and 48 h. Cell vitality was determined by MTT assay. b The expression levels of p-ERK (Thr202/Tyr204), ERK, p-JNK (Thr183/Tyr185), JNK, p-p38 (Thr180/Tyr182) determined by western-blot along with the quantification analysis of p-ERK/total ERK (c), p-JNK/total JNK (d) and p-p38/total p38 (e). Values are expressed as mean ± SD. n = 3.*P < 0.05, **P < 0.01, *** P < 0.001, **** P < 0.0001