Fig. 2

MDA-MB-231 cells were adopted to co-culture with HUVECs. a Cell co-culturing simulations: the MDA-MB-231 cells were co-cultured with HUVECs in indirect cell–cell contacts by a trans-well system. b Quantification of JAG1 expression in the conditioned medium of MDA-MB-231 and MCF7 cells. c Immunoblot results of the expression levels of DLL4, Notch1 and NRP1 in HUVECs. NS no statistical difference