Fig. 5

Overexpression of GPNMB reversed the effect of MALAT1 depletion on cell proliferation and apoptosis in MPP⁺-stimulated SK-N-SH and SK-N-BE cells. a, b MPP⁺-treated SK-N-SH and SK-N-BE cells were transfected with pcDNA3.0-control or pcDNA3.0-GPNMB for 48 h. a The mRNA level of GPNMB was determined by qRT-PCR. b GPNMB protein level was assessed by western blot assay. c–h MPP⁺-treated SK-N-SH and SK-N-BE cells were transfected with si-NC, si-MALAT1-1, si-MALAT1-1 + pcDNA3.0-control, or si-MALAT1-1 + pcDNA3.0-GPNMB, respectively. c, d Cell viability was detected by MTT assay. e Flow cytometry assay was conducted to identify cell apoptosis in vitro. f, g Western blot assay was used to evaluate the expression levels of apoptosis-related proteins, including Bax, Cleaved-casp-3, and Bcl-2. h The protein level of PCNA was detected by western blot. The experiments were repeated three times (n = 3). *P < 0.05 compared to si-NC, ^&P < 0.05 compared to pcDNA3.0-control, ^#P < 0.05 compared to si-MALAT1 + pcDNA-control