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Fig. 5 | Biological Research

Fig. 5

From: Diosmetin inhibits cell proliferation and promotes apoptosis through STAT3/c-Myc signaling pathway in human osteosarcoma cells

Fig. 5

Diosmetin suppressed STAT3 activation and c-Myc expressions in Saos-2. A The expression of STAT3 and phosphorylated STAT3 (p-STAT3) and its downstream protein c-Myc in Saos-2 and U2SO cells detected by western blot at 24 h after diosmetin (0, 10, 30 and 90 μM) treatment. B–E Quantification of related proteins by Image J software. F Diosmetin suppressed the activation of STAT3 in osteosarcoma cells. Saos-2 cells transfected with HA-STAT3 and Flag-STAT3 expression plasmid were either left uninduced or stimulated with 30 μM diosmetin for 24 h, and STAT3 was immunoprecipitated from cell lysates with a Flag-STAT3 antibody, separated by SDS-PAGE, analyzed by western blot with a HA-STAT3 antibody. G Quantification of the proteins from the immunoprecipitation by Image J software. The results are shown as the mean ± SD of three independent experiments (n = 3, *P < 0.05, **P < 0.01 and ***P < 0.001 vs. DMSO control)

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Biological Research

ISSN: 0717-6287

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