Fig. 2

Loss and inhibition of ABCA1 activity impacts migration and invasion abilities of Hs294T cells but not their proliferation. A Representative immunoblots of ABCA1 level of WT and CRISPR/Cas9 generated scrambled and ABCA1 KO cells. Vinculin was analyzed as a loading control (n = 3). B Total cholesterol quantitative measurement of WT, scrambled and ABCA1 KO cells. (n = 9 from three independent experiments, one-way ANOVA with Sidak’s multiple comparisons test). C Esterified cholesterol quantitative measurement of WT, scrambled and ABCA1 KO cells (n = 9 from three independent experiments, one-way ANOVA with Sidak’s multiple comparisons test. D Representative immunoblots of ABCA1 level of Hs294T WT cells treated either with DMSO (vehicle) or 12.5 μM probucol for 2 h. Vinculin was analyzed as a loading control (n = 3). E Total cholesterol quantitative measurement assay of DMSO- or 12.5 μM probucol-treated cells for 2 h (n = 9 from three independent experiments, unpaired Student t-test). F Total cholesterol quantitative measurement of DMSO- or 12.5 μM probucol-treated cells for 16 h (n = 9 from three independent experiments, unpaired Student t-test). G Esterified cholesterol quantitative measurement of DMSO- or 12.5 μM probucol-treated cells for 16 h (n = 9 from three independent experiments, unpaired Student t-test). H, I Quantification of relative proliferation potential after BrdU incorporation assay of scrambled and ABCA1 KO cells (H) or of DMSO- or probucol-treated cells (I) (n = 9 from three independent experiments, unpaired Student t-test). J, K Calculated covered distances of scrambled and ABCA1 KO (J) or DMSO- and probucol-treated cells (K) after 72 h (n = 90 from three independent experiments, unpaired Student t-test). L, M Relative invasiveness of scrambled and ABCA1 KO cells expressed as fold change of scrambled (L) or DMSO- and probucol-treated cells expressed as fold change of DMSO-treated cells (M) after 24 h (n = 9 from three independent experiments, unpaired Student t-test). Data are mean ± SD. ns non-significant, *p ≤ 0.05, ***p ≤ 0.001 and ****p ≤ 0.0001