Fig. 3
Overview of the protein expression profile for the catabolism of 3-HPA and 4-HPA in P. xenovorans strains. Heatmaps show changes in protein expression levels of cells grown until exponential phase on 3-HPA and 4-HPA relative to glucose as sole carbon source. Each value is an average of Log2(expression change) of at least four independent experiments. Significant statistically differences are represented as colored border for the corresponding strain. Peripheral and central pathways for 3-HPA and 4-HPA are depicted including their metabolic intermediates. Catabolic enzymes: MhaAB: 3-HPA 6-hydroxylase; HmgA: HMG 1,2-dioxygenase; HmgB: Fumarylacetate hydrolase; HmgC: Maleylacetate isomerase; HpaBC: 4-HPA monooxygenase; HpaD: HPC 2,3-dioxygenase; HpaE: CHMS dehydrogenase; HpaF: CHM isomerase; HpaG: OPET decarboxylase. Metabolic intermediates of 3-HPA and 4-HPA degradation: HMG: Homogentisate.; MA: Maleylacetoacetate; FA: Fumarylacetoacetate; HPC: Homoprotocatechuate; CHMS: 5-carboxymethyl-2-hydroxy-muconic semialdehyde; CHM: 5-carboxymethyl-2-hydroxy-muconic acid.; OPET: 5-oxo-pent-3-ene-1,2,5-tricarboxylic acid; HHDD: 2-hydroxy-hept-2,4-diene-1,7-dioic acid. TCA cycle: Tricarboxylic acid cycle. ND: Not determined