Fig. 2
From: Cx43 hemichannels and panx1 channels contribute to ethanol-induced astrocyte dysfunction and damage
Ethanol increases the activity of large-pore channels in HeLa cells transfected with Cx43 or Panx1. (A-D) Representative images depicting Cx43EGFP (green), Panx1EGFP (green) and DAPI (blue, 5 µM and 10 min of exposure) labeling by HeLa-Cx43EGFP (A-B) and HeLa-Panx1 EGFP (C-D) cells under control conditions or treated for 24 h with 100 mM ethanol. Insets at the right of each panel depict the respective DAPI labeling alone (top) or plus the phase view merged with EGFP (bottom). (E-F) Time-lapse measurements of DAPI uptake by HeLa-Cx43EGFP (E) and HeLa-Panx1 EGFP (F) cells under control conditions (white circles) or treated for 24 h with 100 mM ethanol (red circles). (G) Averaged DAPI uptake rate (AU/min) normalized with the control condition (dashed line) by parental HeLa, HeLa-Cx43EGFP and HeLa-Panx1 EGFP cells treated for 24 h with 100 mM ethanol. In some experiments, HeLa-Cx43EGFP or HeLa-Panx1 EGFP cells were treated for 24 h with 100 mM ethanol plus 50 µM gap19 or 50 µM 10panx1, respectively. *p < 0.05, **p < 0.01, ethanol treatment compared to control conditions (two-way ANOVA followed by Tukey’s post-hoc test). Data were obtained from at least three independent experiments with three or more repeats each one (≥ 20 cells analyzed for each repeat). Calibration bar = 15 μm