Fig. 1
From: An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig sperm
Comet assay Olive Tail Moment (OTM) after incubation of sperm with different concentrations (0 mM, 0.1 mM, 1 mM, 5 mM and 50 mM) of Mn2+/Ca2+ (A) or Mg2+/Ca2+ (B) in non-permeabilized (−TX) and permeabilized (+ TX) samples. Different letters indicate significant differences (P ≤ 0.05) between concentrations of Mn2+/Ca2+ (A) or Mg2+/Ca2+ (B) within non-permeabilized (−TX) and permeabilized (+TX) samples. Comparisons between non-permeabilized (−TX) and permeabilized (+TX) samples, or between ions (Mn2+/Ca2+ vs. Mg2+/Ca2+) are not depicted