Table 1 Olive Tail Moment (OTM) after incubation with different concentrations of Mn2+/Ca2+ or Mg2+/Ca2+ (0 mM, 0.1 mM, 1 mM, 5 mM and 50 mM) in non-permeabilized (−TX) and permeabilized (+TX) samples
From: An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig sperm
DNA damage (olive tail moment) | ||
|---|---|---|
Non-permeabilized (−TX) | Permeabilized (+TX) | |
(A) Mn2+/Ca2+ | ||
Control (0 mM) | 22.72 ± 5.18a | 28.73 ± 4.65a |
0.1 mM | 34.03 ± 5.88b | 29.55 ± 4.84a |
1 mM | 41.45 ± 3.81c | 39.45 ± 4.41b |
5 mM | 58.07 ± 7.38d | 51.01 ± 5.02c |
50 mM | 60.53 ± 6.93d | 64.41 ± 8.17d |
(B) Mg2+/Ca2+ | ||
Control (0 mM) | 34.48 ± 7.86a | 35.20 ± 7.20a |
0.1 mM | 34.93 ± 8.22a | 35.99 ± 5.90a |
1 mM | 39.06 ± 11.26a | 37.21 ± 6.32a |
5 mM | 39.08 ± 6.61a | 46.63 ± 8.01b |
50 mM | 45.18 ± 5.94c | 50.35 ± 4.89b |