Table 2 Olive Tail Moment (OTM) after incubation with 10 mM Mn2+/Ca2+ (A) or 10 mM Mg2+Ca2+ (B) for different incubation times (0 min, 2 min, 10 min, 30 min, 60 min), in non-permeabilized (−TX) and permeabilized (+TX) samples
From: An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig sperm
DNA damage (olive tail moment) | ||
|---|---|---|
Non-permeabilized (−TX) | Permeabilized (+TX) | |
(A) 10 mM Mn2+/Ca2+ | ||
Control (0 min) | 22.57 ± 4.82a | 28.73 ± 4.65a |
2 min | 63.80 ± 3.80b | 60.07 ± 2.82b |
10 min | 60.71 ± 4.55b | 57.85 ± 2.39b |
30 min | 58.34 ± 5.24b | 59.55 ± 3.52b |
60 min | 60.42 ± 6.03b | 59.59 ± 3.33b |
(B) 10 mM Mg2+/Ca2+ | ||
Control (0 min) | 34.48 ± 7.86a | 35.20 ± 7.20a |
2 min | 37.44 ± 6.01a | 38.35 ± 7.67a |
10 min | 40.16 ± 6.29a | 37.04 ± 6.75a |
30 min | 37.76 ± 5.24a | 34.30 ± 15.30a |
60 min | 36.63 ± 7.79a | 47.51 ± 13.27b |