Table 4 Percentages of motile sperm, viable sperm, sperm with high intracellular ROS, sperm with high intracellular superoxide levels and sperm agglutination after incubation with different concentrations of Mg²⁺/Ca²⁺ (0 mM, 0.1 mM, 1 mM, 5 mM, 10 mM and 50 mM) and for different incubation times (0 min, 2 min, 10 min, 30 min, 60 min)

81.69 ± 6.36^a

50.93 ± 12.74

8.01 ± 6.28

36.95 ± 13.67

7.48 ± 2.05^a

75.59 ± 8.97^a

53.82 ± 13.40

8.30 ± 5.73

32.18 ± 14.13

10.85 ± 3.35^a

76.12 ± 10.89^a

56.87 ± 12.12

7.01 ± 6.31

32.42 ± 9.04

17.18 ± 5.10^a

67.32 ± 9.64^a

55.09 ± 6.24

11.50 ± 7.77

40.16 ± 19.29

36.70 ± 19.72^a

74.28 ± 5.60^a

44.76 ± 8.49

14.25 ± 11.05

56.46 ± 13.32

59.09 ± 6.82^b

5.02 ± 1.89^b

38.31 ± 7.94

12.61 ± 7.60

46.77 ± 8.64

84.77 ± 5.56^b

83.49 ± 5.29^a

54.70 ± 16.13

8.38 ± 3.84

46.28 ± 19.39

8.81 ± 1.44^a

78.16 ± 10.8^a

58.95 ± 16.71

11.90 ± 5.12

38.04 ± 13.45

10.41 ± 4.47^a

73.11 ± 11.92^a

60.16 ± 14.58

12.23 ± 5.23

49.00 ± 18.74

15.11 ± 2.44^a

65.59 ± 2.35^b

47.63 ± 10.77

13.56 ± 7.69

61.78 ± 5.44

34.24 ± 7.84^a

70.74 ± 4.18^b

48.81 ± 16.93

12.80 ± 4.90

64.50 ± 9.59

57.82 ± 4.85^b

6.19 ± 1.84^c

47.90 ± 6.50

6.34 ± 3.22

49.68 ± 6.26

86.15 ± 3.89^b

83.47 ± 7.34^a

60.98 ± 11.23

6.46 ± 2.40

34.65 ± 15.04

11.02 ± 1.43^a

75.67 ± 7.75^a

63.44 ± 8.67

9.87 ± 2.33

32.02 ± 14.21

14.80 ± 3.63^a

71.39 ± 13.43^a

58.29 ± 10.18

9.85 ± 2.68

36.89 ± 15.05

20.17 ± 5.94^a

65.06 ± 4.57^a

55.97 ± 9.46

12.26 ± 4.55

43.39 ± 16.35

34.56 ± 5.70^b

66.69 ± 2.99^a

42.57 ± 26.12

12.07 ± 3.73

45.16 ± 18.95

61.23 ± 5.97^b

4.14 ± 1.74^b

38.20 ± 9.45

6.04 ± 2.72

39.00 ± 10.36

81.44 ± 2.36^c

75.29 ± 11.65^a

49.09 ± 17.94

4.91 ± 4.41

45.43 ± 11.86

19.00 ± 12.95^a

74.82 ± 11.46^a

42.00 ± 20.10

5.00 ± 5.68

49.88 ± 9.71

19.35 ± 5.11^a

65.67 ± 17.99^a

50.38 ± 11.28

5.31 ± 5.31

53.14 ± 6.49

24.11 ± 6.06^a

67.04 ± 0.97^a

44.39 ± 16.67

5.64 ± 5.67

63.49 ± 8.07

33.15 ± 6.93^a

59.06 ± 5.92^a

31.52 ± 15.86

4.68 ± 3.55

65.53 ± 24.71

56.22 ± 11.57^b

4.32 ± 1.71^b

33.24 ± 5.47

3.12 ± 2.97

56.88 ± 16.61

77.70 ± 7.48^c